| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020195 |
| Chromosome: | chromosome 10 |
| Location: | 4049842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447735 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGTGCACGGCCCGCATGGGGACCACCAGCTGCCGCACGCCGCCAAAC |
| Internal bar code: | TGTTTTTCTCGCTCCCCTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2750 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATGCCTGCCGCTCATCT |
| Suggested primer 2: | CACCTTGACCGTCTTCGTCA |