Insertion junction: CLIP2.020203_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020203
Chromosome:chromosome 3
Location:5516940
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g184900 (1 of 1) IPR027417//IPR029060 - P-loop containing nucleoside triphosphate hydrolase // PIN domain-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGGGCCGACTGCGCCGCGACGGCGCCGCCGTGTACCCAGACTACGACG
Internal bar code:TTCTAAATAGGAGGGTCCGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:964
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGATGCGCTGTGCTAAC
Suggested primer 2:GAGAAAGTTTGCGCGTGTGT