| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020204 |
| Chromosome: | chromosome 7 |
| Location: | 4877040 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346350 | (1 of 2) PTHR10174//PTHR10174:SF113 - RETINALDEHYDE BINDING PROTEIN-RELATED // RETINALDEHYDE-BINDING PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCAGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT |
| Internal bar code: | AACCGATGGTAGGAGGTAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 76.1905 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGTGTGTGTTCGTGTG |
| Suggested primer 2: | ATCCCTTTCAAGCCTCCAGC |