Insertion junction: CLIP2.020206_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020206
Chromosome:chromosome 16
Location:4325580
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g668950 CPZ (1 of 1) 3.4.17.1 - Carboxypeptidase A / Carboxypolypeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCGATACCGGGCAGGCGGGCGTCATGCATATGGAGAGTGAGGGCGT
Internal bar code:GCGCTATACGCGTGTTGGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1938
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCAACCGCCAGGAAATG
Suggested primer 2:ACGGGTGTGTGGGAATCATC