Insertion junction: CLIP2.020226_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020226
Chromosome:chromosome 10
Location:1486932
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g428600 (1 of 13) PF00646 - F-box domain (F-box) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGGTGGGCGGCTGGCCCGGACCGCAGCGGGCTGTGGCACTACCTGTC
Internal bar code:GCAGGGTGCGTGCGAGCCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2273
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCAAACCCGTTCCAATGT
Suggested primer 2:TGTCTTCACCGCTGTTCTCC