| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020230 |
| Chromosome: | chromosome 14 |
| Location: | 1931055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620400 | SPC22 | Signal Peptidase, 22 kDa catalytic subunit; (1 of 1) K12948 - signal peptidase complex subunit 3 (SPCS3, SPC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCGCCGGTGTAGCACCGGTGTGAGACCAACCAGAGCCAGCGCCCGGC |
| Internal bar code: | TGAATAGGGGGCTAGGTTTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2962 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACAGTAGTTGGCACGT |
| Suggested primer 2: | ACAGCATTCCACACCGCATA |