Insertion junction: CLIP2.020246_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020246
Chromosome:chromosome 3
Location:3336355
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166000 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTACAGCGTATCCTCGTCGAGGCGGGCTCGGATGGATTTGGAGGTTATTC
Internal bar code:TTGCGATCGGATTACTGAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACAGGTCGTTCAGCTTC
Suggested primer 2:CCATAGCGCATTTCCACACG