| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.020266 |
| Chromosome: | chromosome 12 |
| Location: | 4327653 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518900 | AKR9 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Aldo/keto reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCGCCAAACTGCTTGGTCACGTTCCTGTGGGGCGGCGCAAGGGGCCA |
| Internal bar code: | CCTGAGACCTGCTCCGCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGATGGTGCAGTACTG |
| Suggested primer 2: | CTCCTTGTCCTCCACACCAC |