| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020267 |
| Chromosome: | chromosome 5 |
| Location: | 645681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800523 | (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGATCAGAGGGAGGCGGCAACGACAATCACAGCCGCCGCGGCAGCGGCG |
| Internal bar code: | CTGCTTTACGATAGGACAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3354 |
| LEAP-Seq percent confirming: | 76.7442 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGAAGCTCGGGGTCTGTC |
| Suggested primer 2: | CCGTGTGACTACGTGGACAA |