Insertion junction: CLIP2.020268_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020268
Chromosome:chromosome 10
Location:3931009
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g446700 ANK28 (1 of 2) PTHR24161:SF17 - PALMITOYLTRANSFERASE; Predicted protein with ankyrin repeats intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTATGCAAATGCCGTCCTAATGCTGCGCCACCTCCATTGCGGCCCAA
Internal bar code:TTCACCTACTCATTTAAATATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1236
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGTTGTCGCGCTTATG
Suggested primer 2:TGTGGACAGTCCTGACAACG