Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.020270 |
Chromosome: | chromosome 7 |
Location: | 3310187 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g334650 | (1 of 1) K10695 - E3 ubiquitin-protein ligase RNF1/2 [EC:6.3.2.19] (RNF1_2) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACAAGCCTCTAGACGGCCTGAGCTGCTGCTCTCTGCCCTGTCCTCCT |
Internal bar code: | GTAATGGTATTTTGCTAACAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2073 |
LEAP-Seq percent confirming: | 97.619 |
LEAP-Seq n confirming: | 41 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTACCCTTTCGGCCCTCAAC |
Suggested primer 2: | ATTAGCGGGTATTCGGCCAG |