Insertion junction: CLIP2.020283_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020283
Chromosome:chromosome 10
Location:1726003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430100 ELG22 Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACAGTCCCGTCATTGCGCCACCCAGCTCCCACGCGCACAAGTGCCCA
Internal bar code:AGTTGTAGCGAAATCCCCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:656
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTACCCAGCAACAGTCT
Suggested primer 2:CATCGCAATACCCAACGCTG