| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020285 |
| Chromosome: | chromosome 2 |
| Location: | 2851753 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094700 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGTCCAATCGGCTGTGCGGGCTCTACAGCTACCAACCTAAAATGGAT |
| Internal bar code: | ATGTCGCAGGACTTCGCCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4483 |
| LEAP-Seq percent confirming: | 99.0385 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACTGTACACACACGCCC |
| Suggested primer 2: | GCACGTGCACCATGATGATC |