Insertion junction: CLIP2.020293_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020293
Chromosome:chromosome 2
Location:1708107
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086000 ARS8 Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGACTCACCCCGGGCTGCGGAGTTGCTGGTGATGTTGGTGTTGTGGCA
Internal bar code:CCTTCCGGCAAGACCGACATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2159
LEAP-Seq percent confirming:92.9412
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGATGCCAAGTACATGC
Suggested primer 2:CGTGTGGGACAGATGGTGAA