Insertion junction: CLIP2.020307_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020307
Chromosome:chromosome 2
Location:3709297
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g100500 CYG22 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGAATTAGTATAGTGTTGCGAAACACCCGCACTTACCCATCGATCGCT
Internal bar code:TATGAGAAACCTGCCACTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4575
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TAAACAGGGCGCCATCAACT
Suggested primer 2:GCATGCGGAAGATCGTGAAC