Insertion junction: CLIP2.020325_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020325
Chromosome:chromosome 6
Location:3117262
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275450 (1 of 1) PF00069//PF13855 - Protein kinase domain (Pkinase) // Leucine rich repeat (LRR_8) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTTGCGCGGCGCCAGCAGCACCTGCCAGGCATGACGTGGTGTGTGTG
Internal bar code:CCAGGAATTGCAGATTAGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1848
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GATCCAGCTTCATCCGAGGG
Suggested primer 2:CGTGAGAAGGAAGCTTGGGT