Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.020349 |
Chromosome: | chromosome 16 |
Location: | 1393564 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g652150 | FBP4 | (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGGGTGGCTTTAGTAGAATCGCGCCTGCATGCATGGGTGGCGTAGC |
Internal bar code: | GGCGGGTCCTTGGAGCGGATAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2797 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 89 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGGGAGTGAAAACGACAGA |
Suggested primer 2: | TAATCAGTCACACGGCACCC |