Insertion junction: CLIP2.020383_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020383
Chromosome:chromosome 10
Location:2113296
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433100 OBGE1,OBGE Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGTGCGTAGTGTGATGGCGTGTGTGCCTAGATGAGCACAAGCAGGAC
Internal bar code:GCTGGCTTAAAAGTTATTTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTATCCTTGAGGTCCGGG
Suggested primer 2:GCGATGCGTTTGTACAGGTC