| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020393 |
| Chromosome: | chromosome 1 |
| Location: | 5575054 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039702 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGTCAGGTTTTGGGGCTGAGCCAGTCGGTGCATTTTTTGCATGGAG |
| Internal bar code: | GTTCTGTTGTGGGGTGTAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2565 |
| LEAP-Seq percent confirming: | 98.8095 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACCCAGTGTCCGCATGT |
| Suggested primer 2: | CAGGTAGTAGCTCTGGCGTG |