Insertion junction: CLIP2.020422_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020422
Chromosome:chromosome 8
Location:1894495
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367600 ASL1,CYSL,CYSM,OASTL1 (1 of 3) 2.5.1.47 - Cysteine synthase / OAS sulfhydrylase; O-acetylserine (Thiol)-lyase/cysteine synthase 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGTACCCGGCTGTACGGCACAGCGGTTTCATTCCGCGGGACAACATCA
Internal bar code:CACTGTGGTAGTCAGTGCTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:94
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCGTGTCCTATCATGAG
Suggested primer 2:CAGTACTCTAGCTGCCTCGC