| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020472 |
| Chromosome: | chromosome 14 |
| Location: | 375054 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610300 | (1 of 88) 3.6.4.12 - DNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGAGGGAGCATTAGCCTCCGGCCACAGTCCCAATGGAAGCTAGATGC |
| Internal bar code: | CGGCTTCCCCTGGTCTGCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAGCGCCTGACTCACAT |
| Suggested primer 2: | ACTAGTTGCGTGTCCGTGAG |