Insertion junction: CLIP2.020492_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020492
Chromosome:chromosome 7
Location:750855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g317750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCACGCAACCCTGCGACCCCCCGCACCATCCATGTTGCGATGTCGCCC
Internal bar code:TTGATCGACTCCAAACTTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1767
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:GGTACGGTGCTCTACGGATG
Suggested primer 2:TAGGAGTGTGTGGCTTGCTG