Insertion junction: CLIP2.020495_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020495
Chromosome:chromosome 3
Location:4669457
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g177150 (1 of 2) K17263 - cullin-associated NEDD8-dissociated protein 1 (CAND1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGTTGGGCCGGATCCAAAGGCTGAGGCCCGAGGCGCCCGCGAACGGC
Internal bar code:GGCTCCCCAAGATGTGTAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2248
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCAGCTAGGATCGATTGC
Suggested primer 2:CGTTCACGTTTTGCAGCTCA