Insertion junction: CLIP2.020496_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020496
Chromosome:chromosome 17
Location:1966141
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g710200 IAD1 Indoleamine 2%252C3-dioxygenase; (1 of 1) 1.13.11.11 - Tryptophan 2,3-dioxygenase / Tryptophanase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCAGCTTGTGCACGGAGGAAGCGGCACACGACCACGGTGCGCGCCGCC
Internal bar code:GAGTACTCAGGCATAAAAGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1402
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTATCAGTGTGCGTTCCC
Suggested primer 2:GTAGCAGCATTAGCAACGGC