| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020496 |
| Chromosome: | chromosome 17 |
| Location: | 1966144 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710200 | IAD1 | Indoleamine 2%252C3-dioxygenase; (1 of 1) 1.13.11.11 - Tryptophan 2,3-dioxygenase / Tryptophanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGTTAGCCGCGCTGCTCGATGCCGTCGGGGAGCATAGGATTAGGAGC |
| Internal bar code: | GAGTACTCAGGCATAAAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCAGCATTAGCAACGGC |
| Suggested primer 2: | TGGTATCAGTGTGCGTTCCC |