| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.020499 |
| Chromosome: | chromosome 5 |
| Location: | 2450555 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238900 | HEL25 | (1 of 1) K14779 - ATP-dependent RNA helicase DDX52/ROK1 [EC:3.6.4.13] (DDX52, ROK1); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCTGCCAGGGTTGGGCCCAGCAGAATTGCTTGGCGCTCGGCGCTCAG |
| Internal bar code: | AGGGGTGAATCGGACGCTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1354 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCTGTCTCCCTCTGAT |
| Suggested primer 2: | GCTTCATGGAGCAGGTGAGT |