Insertion junction: CLIP2.020515_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.020515
Chromosome:chromosome 10
Location:4418540
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g450450 FAP18 Flagellar Associated Protein 18; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACAGAGGTGGAGCCCACTGCCTCTGACTTCGCCGACTTGACGGAGGTG
Internal bar code:CGCCCTGTTTCATATCCCTATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2623
LEAP-Seq percent confirming:98.3607
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCATGTTGTTCCGTCGA
Suggested primer 2:CAGCTTGTAGGTGCCACTGA