| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.020518 |
| Chromosome: | chromosome 12 |
| Location: | 9774257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550277 | MRS2 | Mg2+ transporter protein, CorA-like and putative mitochondrial splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGACACACCCTGGGGACACACCTGGCCGAAGGGGTGCAGCAGACCG |
| Internal bar code: | CTTTCGTAACAATCGTCTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1089 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCATCCGACACAACCCA |
| Suggested primer 2: | GTGTGAAGTGACAACGGTGC |