Insertion junction: CLIP2.020527_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020527
Chromosome:chromosome 1
Location:6980727
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g049826 PHC59 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATTCGTTGACAAGTGCTCTGCCTCTCACAGGAAAAGTGGGCTGCGCC
Internal bar code:ACCATCCCTGATTCGGGTGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGCCCTACCTTCTCATCA
Suggested primer 2:AGGTTAGGCAGATTGGGCAC