| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.020540 |
| Chromosome: | chromosome 12 |
| Location: | 396395 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495401 | POC12 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGAGTGCCAGTTCGTTTACATACGGAGGCCCATGCCCGGCAGAGC |
| Internal bar code: | GTAGCCTTTTGGGGTATCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 64.7059 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGTCCCTCTTCCCAAG |
| Suggested primer 2: | GGCACCGTCCAAAACAACTC |