Insertion junction: CLIP2.020553_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020553
Chromosome:chromosome 11
Location:1109370
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467675 (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGTCGAGATTTCTTGACGATGACCCGATGTGGCTGCGGCAAAACGAC
Internal bar code:GGGCCCAGGGCTGGGGGGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1940
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACAGAGGAGGTGGAGGA
Suggested primer 2:ATTCGCCTGCCATGGTGTAA