| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020556 |
| Chromosome: | chromosome 5 |
| Location: | 2640298 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240100 | EXN9 | 3' repair exonuclease; (1 of 3) 3.1.11.2 - Exodeoxyribonuclease III / Exonuclease III | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCTGGCGGAACAAGCGCATGCGCATCTGCAGCCTATTCTGTGCGCG |
| Internal bar code: | GTGGGTAATGCCTAACGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1648 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGTTGAGGTGCTAGAC |
| Suggested primer 2: | CGAACAGTTGTTGCAGGGTG |