| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.020564 |
| Chromosome: | chromosome 12 |
| Location: | 5605094 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531200 | FOX2 | Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) | 3'UTR |
| Cre12.g531250 | (1 of 1) K00480 - salicylate hydroxylase (E1.14.13.1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTGATGCGCGACATGCCTCGCTGCTTAGGCGAGACCTCTTGCCTACG |
| Internal bar code: | TCCGTAGTCCACAGTTTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2279 |
| LEAP-Seq percent confirming: | 78.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCAGCAACTGTGTGAG |
| Suggested primer 2: | AACGACACAATGGTCTCCCC |