Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.020576 |
Chromosome: | chromosome 4 |
Location: | 3539572 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g228300 | STK | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX); Serine/threonine protein kinase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACGCAGCCGGCCAGGCCCGCGGCAAACAGAGCAGCAAACCCCTGTGC |
Internal bar code: | TCGGCGACATGACTGACAAATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3685 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 20 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTTAGCTGCGGGTTAGCA |
Suggested primer 2: | GAACCCACTTGGAACGCAAC |