Insertion junction: CLIP2.020576_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020576
Chromosome:chromosome 4
Location:3539575
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g228300 STK (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX); Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGGCGCGTGCGCTGTTGGCATGTGTACATACGGTATGCATCTGTGTCT
Internal bar code:TGGTTATTTAGGGTTGACATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:5530
LEAP-Seq percent confirming:93.4426
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCACTTGGAACGCAAC
Suggested primer 2:TTGTTAGCTGCGGGTTAGCA