Insertion junction: CLIP2.020577_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020577
Chromosome:chromosome 11
Location:2620794
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGTGAGGGTGGCGCGGCGACGGGCGCTCACCCCACGACCGCGTTGC
Internal bar code:AGGATCCTTGCTTGTAGATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:223
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTCGTAGCAGCAGTAGA
Suggested primer 2:TAGAGCACTTTCGCGGTTGT