| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020590 |
| Chromosome: | chromosome 6 |
| Location: | 2426876 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268800 | FLA17,IFT139,FAP60 | Intraflagellar transport protein 139; (1 of 1) PTHR14699//PTHR14699:SF0 - STI2 PROTEIN-RELATED // TETRATRICOPEPTIDE REPEAT PROTEIN 21 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTTGCGGGTTTCCGGACGACCGTAATCATGCCTGGAACTTCCCTCCC |
| Internal bar code: | CCAACAAGTTACTGGAAACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATCCCCCACCATCTCAT |
| Suggested primer 2: | GACATGATAAGGGAGGGGCG |