Insertion junction: CLIP2.020606_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020606
Chromosome:chromosome 16
Location:4996722
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686200 TSPSP2,TPT1,TPS3,TSSP2 (1 of 2) K16055 - trehalose 6-phosphate synthase/phosphatase (TPS); Triose-phosphTrehalose-6-phosphate synthase/phosphataseate transporter, class II intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCAACAAGGGGCGGGGGCAGCAGTGGGGCTGCGGCGCCGTGGCAGTG
Internal bar code:GGTGACTCTTCATCATGGCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:468
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTAATCCCCCTCCCCTC
Suggested primer 2:CGTCTCTTTGTCCCTTCCCC