| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.020615 |
| Chromosome: | chromosome 13 |
| Location: | 2595133 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580700 | DAL2 | allantoate deiminase; (1 of 1) 3.5.3.9 - Allantoate deiminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTTCAGCAGCTTGGAATCTTCGAATTGCCTGCGCGGTGCCTGCACC |
| Internal bar code: | TGCTTAATCCGGGGAACCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGCGTATGTCCACACT |
| Suggested primer 2: | GATGGGTGCGATGCAATCAC |