Insertion junction: CLIP2.020632_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.020632
Chromosome:chromosome 9
Location:4784237
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g402441 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTTAAGGCCAAACCTCCTCCGCGCGCTGTGAATTACATACAAACGCTC
Internal bar code:CGTTCATGGTCCGATCGAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3823
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGTGTTTCTGCTTGACG
Suggested primer 2:GTTGGTGCTACCGATGGGAA