Insertion junction: CLIP2.020639_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020639
Chromosome:chromosome 12
Location:7854968
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553700 PDO2 Protein disulfide oxidoreductase; (1 of 3) PTHR13887//PTHR13887:SF10 - GLUTATHIONE S-TRANSFERASE KAPPA // FRNE PROTEIN-LIKE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGAGGAGCTGTTCGAGCAGATCTGCAGCAAGTAAGTGCTGAGCTGCAT
Internal bar code:TAAATGAAGGGTGGCATGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1395
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGGTCTGGCACTTTGTTT
Suggested primer 2:GGTTGGGGAGATTGGGAAGG