Insertion junction: CLIP2.020641_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.020641
Chromosome:chromosome 2
Location:935616
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079300 VPS4 AAA-ATPase of VPS4/SKD1 family; (1 of 1) K12196 - vacuolar protein-sorting-associated protein 4 (VPS4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCATGCTGCCATAATCCCCCATCTTCAGGAGGACAACAAGGAGAAGG
Internal bar code:AGCAATTATTACTGGCGATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3842
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAAGATCACTGCCTCCTT
Suggested primer 2:CGCTTACGCTCTGGACCTAG