| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020652 |
| Chromosome: | chromosome 7 |
| Location: | 419498 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315000 | CSG6 | Chlamydomonas specific family G protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGAAGTACAGCTTGCCGCGCAGCGCCAGCTCCAGCTGCAGCGGCTGC |
| Internal bar code: | GGCGTGGGCTCTGTAGTGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1290 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCAGGGGTTGGGAGGAGG |
| Suggested primer 2: | GCATAAGCAACGTAGCACCG |