Insertion junction: CLIP2.020673_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.020673
Chromosome:chromosome 2
Location:5522122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g114750 CDKP6,CDPK6,CDPK4 (1 of 6) PTHR24349//PTHR24349:SF23 - SERINE/THREONINE-PROTEIN KINASE // MAP KINASE-ACTIVATED PROTEIN KINASE 5; Calcium/calmodulin-dependent protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAATGGCGTAGTAGAGCGCGCTGCGCACACAGCAACGCAAGTGCAAGT
Internal bar code:GAATTACACAGGGTCATCAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:926
LEAP-Seq percent confirming:39.7436
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCCGCGGCAAAATATTC
Suggested primer 2:AAGGCCATGTACAGGTGCTC