Insertion junction: CLIP2.020683_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.020683
Chromosome:chromosome 6
Location:7927533
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g304100 (1 of 1) K17261 - adenylyl cyclase-associated protein (CAP1_2, SRV2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCGCTGCGCTCGCACGCACCACGCCCACTCTTCACAGCTGACCGCCG
Internal bar code:ACCATGATTTGTATTATGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTTTGTGACTGCCCCCT
Suggested primer 2:AGTCGATGGTGCAGAAACGT