| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.020692 |
| Chromosome: | chromosome 11 |
| Location: | 627658 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467611 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAGGAATGCAGCCGCTGTCACGGCCGCCCGAAACCGTGCCTGGCTTT |
| Internal bar code: | AACTCAATGAGAAAGTTGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCCGATGTGTTTTGCT |
| Suggested primer 2: | AATATCCACAACCCGCAGCA |