| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020697 |
| Chromosome: | chromosome 2 |
| Location: | 6850874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388430 | (1 of 2) IPR000104//IPR001202 - Antifreeze protein, type I // WW domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACTCACGTTTGGCCTCCATAGCGGCAGCGATGGGGTCCAGCGGCTC |
| Internal bar code: | GGCCTCTTTATTATTCTCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 81.1594 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCTTGCAGTCCTGGAAT |
| Suggested primer 2: | CTCGTCGTCATCGTCTGAGG |