| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020699 |
| Chromosome: | chromosome 9 |
| Location: | 5302334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407350 | NAT23 | N-acetyltransferase; (1 of 4) IPR006917//IPR011256 - SOUL haem-binding protein // Regulatory factor, effector binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGGTTTCTTACCCTGTCCCATCGCATCCTGACACCGATTACCGACTC |
| Internal bar code: | TCATTCCTCGTACACTCGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1761 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGTGGTGTTGTGAGG |
| Suggested primer 2: | ACACACACACACACACACCT |