| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020700 |
| Chromosome: | chromosome 1 |
| Location: | 4574506 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031500 | (1 of 1) 1.10.3.2//1.10.3.3 - Laccase / Urishiol oxidase // L-ascorbate oxidase / Ascorbase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGCCGTCGACGGGGCGCAGGTCGCCGTTGAAGGAGTTGGCAGGGTT |
| Internal bar code: | AGTTCATTGAGATCTAATGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1452 |
| LEAP-Seq percent confirming: | 11.9403 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTACTACGAGGGCCTGA |
| Suggested primer 2: | AGGACACAGTGAAGCCGAAG |