| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.020706 |
| Chromosome: | chromosome 1 |
| Location: | 6133980 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043550 | (1 of 3) IPR000104//IPR004333 - Antifreeze protein, type I // Transcription factor, SBP-box | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGTCATGGTCGCGCGCGCCGACGGCGAACATGCCCCCCAGCAACCCTG |
| Internal bar code: | CGTATTATCGGCGTCGCCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4033 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCTGTGACCATTTGCGC |
| Suggested primer 2: | AGACAACCAATGCGGCACTA |